1. Incubate under anaerobic conditions for the duration of the incubation period. 在厌氧条件下培养，培养时间同培养期。
2. Do not expose reagents to strong light or hypochlorite fumes during storage or during incubation steps. 在存储和孵育过程中不要暴露试剂于强光或次氯酸蒸汽中。
3. Among them, the oocyte preparation, fixation, membrane permeabilization, fat extraction, and antibody incubation are the key steps. 其中，卵母细胞的准备、固定、透膜、脂类抽提、抗体孵育步骤十分关键。
英英解释
n.
1. maintaining something at the most favorable temperature for its development
2. (pathology) the phase in the development of an infection between the time a pathogen enters the body
3. sitting on eggs so as to hatch them by the warmth of the body
